| 1. | Molecular cloning of 5 ' flanking region of ovine keratin associated protein 6 - 1 gene and comparison of the sequences
端调控区的分子克隆及测序结果比较 |
| 2. | The aim of this part is that using bovine as1 - casein 5 ' regulation region and 3 ' flanking region to construct mammary gland expressing vectors
本实验旨在利用牛s1 ?酪蛋白的5 ’调控区和3 ’侧翼区来构建乳腺特异表达载体。 |
| 3. | The research work was divided into 3 parts in this thesis : 1 ) 2 . 5kb 5 " flanking region of the 20ohsd gene was isolated by pcr
本研究工作主要分以下三个部分：第一部分运用pcr技术从大鼠promoterfinder文库中扩增到了2 . 5kb的20 hsd基因5侧翼区序列，并对其进行了序列测定。 |
| 4. | The analysis on the 5 " flanking region revealed tata box , putative api and nur77 response elements , prl and progesterone response elements motifs related with regulation on 20ahsd gene expression
通过软件对克隆到的5侧翼区结构和功能分析，发现了20 hsd基因的tatabox 、 prl反应元件、类固醇激素反应元件、 ap1和nur77等转录因子结合位点。 |
| 5. | In this study , using bioscien tomato as test material and the flanking region of the bioscien insert sequence was determined using inverse pcr and real - time pcr . . by means of verification , this sequence is specific for the transfection event
本研究以华番1号为试材，利用反向pcr和荧光pcr技术已成功将华番1号转基因整合位点dna序列扩增出来。 |
| 6. | These results demonstrated the acbadh promoter is a strong salt - stress - induced promoter . the acbadh 5 ' - flanking region contains two salt - responsive enhancer regions localized between - 1114 and - 892 , - 464 and - 234 and one sliencer region localized between - 892 and - 643
对转基因烟草的gus活性分析表明，在1114和892之间， 464和234之间存在增强子元件，而在892和643之间存在负调控元件。 |
| 7. | Firstly , the bovine as1 - casein 5 ' regulation region was cloned into the vector pegfp - 1 and constructed a new vector p - 5 ; then the bovine asi - casein 3 ' flanking region was inserted into the position of gfp gene in the vector of p - s and a mammary gland universal expressing vector pbcas was created
首先将牛s1 ?酪蛋白的5 ’调控区克隆到载体pegfp - 1上，成为载体p - 5 ；然后将牛s1 ?酪蛋白的3 ’侧翼区克隆到载体p - 5的gfp基因位置，从而构建成乳腺通用表达载体pbcas 。 |
| 8. | ( 3 ) at post - translation level plant mutual sequence of starting translation aaca and eukaryotic secretory signal peptide sequence was added to 5 ' - flanking region of t - pa gene and kdel ( sequence located to endoplastic reticulum ) to 3 ' - flanking region by pcr amplication and plant expression vector pbemt was constructed
( 3 )翻译后水平通过pcr扩增的方式在t - pa基因5端添加了真核分泌信号肽序列和植物翻译起始共有序列aaca ，在3端添加了内质网定位序列kdel ，构建了植物表达载体pbemt 。 |
| 9. | Secondly , the chicken r - ifn cdna was introduced into the vector pbcas between the bovine asi - casein 5 ' regulation region and 3 ' flanking region to create a vector pbcasifn to examine if this bovine asi - casein 5 ' regulation region and 3 ' flanking region could regulate chicken y - ifn to express in the latex by producting transgenic mice
最后，我们将鸡?干扰素cdna插入到载体pbcas的牛s1 ?酪蛋白的5 ’调控区和3 ’侧翼区之间，构建成载体pbcasifn ，以便于通过生产转基因小鼠来检验牛s1 ?酪蛋白的5 ’调控区和3 ’侧翼区是否可以调控外源基因鸡?干扰素在乳腺中特异表达。 |
| 10. | Using transfac 4 . 0 and tssg tssh nsite of softbeny , we can see that a tata box lie in - 188bp - 198bp from transcriptional initiation site , there are several api , c / ebp binding motifs near it or in other 5 " flanking region , a c - fos response element is in - 1133bp - 1143bp from transcriptional initiation site
0软件和softbeny网站上tssg ， tssh ， nsi ’ fe软件共同分析，在a ? ibgp转录起始点上游j到一198区域存在tata盒，在其附近及其5 ’上游远端调节区存在多个api ， c ebp结合位点，并且在转录起始点上游人到一处存在一个c fos结合基序。 |
